معرفی
محلول متوقف کننده الایزا ELISA stop solution
Cat #: DB9690-25ml
Volume: 25 ml
Packeging: Dark glass bottle
Made by DNAbiotech Co. IR Iran
Application Notes:
Each lot of Stop Solution for TMB (3,3'5,5'-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well.
Addition of 50 ul stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm.
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