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Specifications:

SDFA Kit (Sperm DNA Fragmentation Assay) is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. SDFA Kit allows for an improved evaluation of spermquality, complementing the information offered by the conventional sperm analysis. Conventional semen analysis only studies spermatozoa concentration, motility and morphology.  It is an incomplete study, as it omits analysis of one of the most important parameters, the integrity of the DNA molecule. 15% of men considered to be infertile present normal sperm analyses, which makes a deeper assessment of the sample important.SDFA Kit facilitates decision-making for medical professionals regarding which assisted reproduction technique is most appropriate for each couple.

SDFA Kit is based on the SCD (Sperm Chromatin dispersion) technique, Produced by Pars iGene Company, based on a controlled DNA denaturation process to facilitate the subsequent removal of the proteins contained in each spermatozoon.  In this way, normal spermatozoa create halos formed by loops of DNA at the head of the sperm, which are not present in those with damaged DNA.

The images of halos generated with SDFA Kitare highly contrasted and can be evaluated precisely using conventional or fluorescent microscopy. The tails of the spermatozoa are preserved, so it is possible to discriminate them from other cell types that might be present in the ejaculate, such as leukocytes. In addition, SDFA Kit allows the visualization of spermatozoa that contain highly degraded DNA compared to other types of less aggressive damage. This fact is of great importance in the diagnosis and monitoring of varicocele.

Medical professionals use information from SDFA Kit solutions at the start of treatment, supplementing conventional semen analysis. Sperm DNA damage is a multifactorial process. Because it can have multiple causes, fragmentation, oxidation, and vitality analysis is especially recommended for:

• Couples with a history of miscarriages.
• Couples with unexplained infertility for more than 6 months
• Men older than 40
• Use of tight-fitting clothing
• Men with a history of cancer
• Men under treatment with prescription medications
• Men exposed to toxic agents
• Men who have had urogenital infections
• Men with unhealthy lifestyle habits: those who smoke, have an unbalanced diet or are obese.
• Poor quality of the embryo during second cycles of egg donation
• Male factor of unknown cause