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DESCRIPTION:

Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 μg/ml (0.1 to 1 mM)

Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step.

Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes.

High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF.

Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.

Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months