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NOTE:

0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines.

  Each cell line responds to Trypsin-EDTA solution in an specific manner.

 For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact technical Service. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.