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TBE:

 TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA. In molecular biology techniques,TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis.

TAE or TBE, which is best?

Of course, it depends on what you want to do:

• TBE is a better conductive medium than TAE so is less prone to overheating, so use TBE for long runs!
• Borate is an enzyme inhibitor so TBE is not a good buffer to use if you will be isolating the DNA for downstream enzymatic steps. For example, borate carry-over could affect your ligations, so use TAE instead in this situations.
• Acetate gives improved separation of large DNA fragments
• On the other hand, borate resolves <2kb fragments better, so use TBE for smaller fragments
• And finally, TBE is reputed to give nicer bands than TAE (although I’m not sure why – does anyone know?

 Compare this product by TAE buffer by clicking HERE.