بافر TAE حجم 500 سی سی 50X

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بافر TAE حجم 500 سی سی 50X

بافر TAE حجم 500 سی سی 50X

50X TAE buffer, 500 mL

Cat#: DB9614

Made by DNAbiotech, IR Iran

Ready to Use

To make 1x TAE from 50X TAE stock, dilute 20ml of stock into 980 ml of DW.

Applications

• Electrophoresis of nucleic acids in agarose and polyacrylamide gels.
• Used both as a running buffer and as a gel preparation buffer.

Composition of 1x TAE buffer:

■ 40 mM Tris (pH 7.6)

■ 20 mM acetic acid

■ 1 mM EDTA

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TAE Buffer

This buffer is used as both a running buffer and in agarose gel. Its use in denaturing gradient gel electrophoresis methods for broad-range mutation analysis has also been described.

TAE has been used at various concentrations to study the mobility of DNA in solution with and without sodium chloride.

High concentrations of sodium chloride (and many other salts) in a DNA sample retard its mobility. This may lead to incorrect interpretations of the resulting DNA banding pattern.

Compared with TBE buffer, TAE buffer offers advantages in subsequent enzymatic applications for the DNA sample. For example, if a DNA sample is going to be used in a cloning experiment, the step that follows its running on an agarose gel is to ligate to a cloning vector.

A DNA sample from a TAE gel is suitable for this purpose, while DNA from a TBE gel is not, because borate in the TBE buffer is a strong inhibitor for many enzymes.

- Compare this product by TBE buffer by clicking HERE.

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