کیت سنجش پروتئین برادفورد (2500 تست)

معرفی

کیت سنجش پروتئین برادفورد

Bradford Protein Assay Kit 2500 Test

For the rapid, sensitive and accurate measurement of protein in various samples.

Catalog Number: DB0017-2500a

Unit Size: 2500 assays

Storage upon receipt:

· 2-8 °C
· Protect from light

To obtain more information about the kit, click HERE. 

Manufactured by: DNAbiotech Co. IR. Iran

www.kalazist.ir 

Kit Components:

Related produtcs:

Bradford protein Assay kit, Cat#: DB0017-625a

Bradford protein Assay kit, Cat#: DB0017-1250a

BCA protein Assay kit (25 ml, 500 Assay), Cat#: DB9684-25ml

BCA protein Assay kit (50 ml, 1000 Assay), Cat#: DB9684-50ml

BCA protein Assay kit (100 ml, 2000 Assay), Cat#: DB9684-100ml

SDS-PAGE gel preparattion kit, (Cat#: DSK100)

بررسی تخصصی

BRADFORD PROTEIN ASSAY

 History & Theory

 Four spectroscopic methods are routinely used to determine the concentration of protein in a solution. These include measurement of the protein's intrinsic UV absorbance and three methods which generate a protein-dependent color change; the Lowry assay, the Smith copper/bicinchoninic assay and the Bradford dye assay.

The first, UV absorbance, requires that a pure protein with known extinction coefficient be used, in a solution free of interfering (UV absorbing) substances. The Lowry and copper/bicinchoninic assays are based on reduction of Cu²⁺ to Cu¹⁺ by amides.  Although this makes them potentially quite accurate, they require the preparation of several reagent solutions, which must be carefully measured and mixed during the assay.  This is followed by lengthy, precisely timed incubations at closely controlled, elevated temperatures, and then immediate absorbance measurements of the unstable solutions.  Both assays may be affected by other substances frequently present in biochemical solutions, including detergents, lipids, buffers and reducing agents. This requires that the assays also include a series of standard solutions, each with a different, known concentration of protein, but otherwise having the same composition as the sample solutions.

The Bradford assay is faster, involves fewer mixing steps, does not require heating, and gives a more stable colorimetric response than the assays described above.  Like the other assays, however, its response is prone to influence from non protein sources, particularly detergents, and becomes progressively more nonlinear at the high end of its useful protein concentration range. The response is also protein dependent, and varies with the composition of the protein. These limitations make protein standard solutions necessary.

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