پانسو اس 5 گرمی Ponceau S

معرفی

پانسو اس 5 گرمی Ponceau S

Cat #: 9721-5gr

CAS no.: 6226-79-5

Content: 5 gr

Chemical Formula: C₂₂H₁₆N₄O₁₃S₄

MW: 672.63 g/mol⁻¹

^{For research use ohly}

Application

Ponceau S solution has been used for membrane staining (Western blotting)

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Description

Ponceau S is the sodium salt of a diazo dye.

Ponceau S solution is a suitable reagent for use in electrophoresis studies.

The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting).

A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.