بافر ریپا (RIPA Buffer) حجم 10ml

معرفی

بافر ریپا (RIPA buffer) حجم 10ml

Cell lysis reagent

Brand: DNAbiotech

Cat #: DB9719

Storage: -20^o C

Packege: Dark brown glass bottle

Volume: 10 ml

Suitable for: WB, ELISA, SDS-PAGE, IP

500 ul ot DNAbiotech RIPA buffer is suitable for lysis of 10.000.000 cell.

To see complete datasheet CICK HERE.

www.kalazist.ir

 Related Products:

PMSF 1 gr (DB9662-1)

PMSF 5 gr (DB9662-5)

بررسی تخصصی

Total Procedure

1. Prepare RIPA Lysis buffer by adding PMSF or your own desired  protease inhibitors to the solution
2. Pour off media from tissue culture dish into waste container
3. Wash cells twice with PBS pouring excess off into waste beaker
4. Carefully soak up any extra PBS with an absorbent.
5. Add 500 ul of RIPA lysis buffer to the culture dish.() 
6. Use cell scraper to scrape cells from the bottom of the dish.
7. Pass cell lysate through pipette 20 times or by vortexing to form homogeneous lysate.
8. Transfer lysate to 1.5 ml microcentrifuge tube.
9. Allow samples to stand for 5 mins in ice or at 4^oC (cold room).
10. (Optional) repeat step 7-9 againe.
11. Centrifuge the resulting mixture at 14,000g for 15 mins in ice or at 4^oC to separate cell debris from protein.
12. Transfer supernatant to a new tube and store at -20^o C.