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Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. ... The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process.

 The main principle involved in column chromatography is adsorption of the solutes of a solution through a stationary phase and separates the mixture into individual components. This is based on the affinity towards the mobile phase and stationary phase.

Column Chromatography is a preparative technique used to purify compounds depending on their polarity or hydrophobicity. In column chromatography, a mixture of molecules is separated based on their differentials partitioning between a mobile phase and a stationary phase